T-cell receptor transfer into human T cells with ecotropic retroviral vectors
نویسندگان
چکیده
منابع مشابه
Functional human antigen-specific T cells produced in vitro using retroviral T cell receptor transfer into hematopoietic progenitors.
In vitro production of human T cells with known Ag specificity is of major clinical interest for immunotherapy against tumors and infections. We have performed TCRalphabeta gene transfer into human hemopoietic progenitors from postnatal thymus or umbilical cord blood, and subsequently cultured these precursors on OP9 stromal cells expressing the Notch human ligand Delta-like1. We report here th...
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The steadily increasing availability of human embryonic stem (hES) cell lines has created strong interest in applying available tools for gene transfer in murine cells to human systems. Here we present a method for the transduction of hES cells with ecotropic retroviral vectors. hES cells were transiently transfected with a construct carrying the murine retrovirus receptor mCAT1. Subsequently, ...
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Retroviral vectors are useful for efficient, stable and single copy transfer of foreign genes into animal cells. However, the low risk murine ecotropic vectors can only be used with rodent cells due to their restricted host range. When using cells from other species, in particular human, amphotropic retroviral vectors and the corresponding packaging cell lines are required. Given the wide host ...
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The successful application of human gene therapy protocols on a broad clinical basis will depend on the availability of in vivo cell-type-specific gene delivery systems. We have developed retroviral vector particles, derived from spleen necrosis virus (SNV), that display the antigen binding site of an antibody on the viral surface. Using retroviral vectors derived from SNV that displayed single...
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T-cell receptor (TCR) gene transfer for cancer immunotherapy is limited by the availability of large numbers of tumor-specific T cells. TCR alpha and beta chains were isolated from a highly lytic HLA-A2-restricted cytotoxic T lymphocyte (CTL) clone recognizing the melanoma-associated Melan-A/MART-1 antigen and inserted into a lentiviral vector carrying a bidirectional promoter capable of robust...
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ژورنال
عنوان ژورنال: Gene Therapy
سال: 2014
ISSN: 0969-7128,1476-5462
DOI: 10.1038/gt.2014.25